Isolation of tonsillar B-cell subsets based on CD77/CD10/IgD staining. Purified tonsillar B cells were triple-stained with FITC-conjugated anti-CD77, PE-Cy5–conjugated anti-CD10, and PE-conjugated anti-IgD mAbs to identify CD77⁺ centroblasts, and from the CD77⁻ fraction, CD10⁺IgD⁻ centrocytes, CD10⁻IgD⁺ non-GC cells (predominantly naive B cells), and CD10⁻IgD⁻ non-GC cells (predominantly memory B cells) and then separated using the indicated sort gates. Isolated B-cell subsets were subsequently reanalyzed to determine the sort purities. Shown are representative data from donor CCT13.