EBV genome loads in tonsillar B-cell subsets isolated by alternative sorting strategies. Duplicate samples from 2 chronic carrier tonsils (CCT12, 13) and from 3 IM tonsils (IMT3, 6, 8) were sorted independently using 2 different protocols. For each tonsil, (1) the top 3 bars indicate EBV genome loads in subsets isolated by CD38/IgD/CD27 sorting into purified naive (CD38−, IgD+,CD27−, □), isotype-switched memory (CD38−, IgD−, CD27+ gray bars), and CD38+ (■) B-cell subsets, and (2) the bottom 4 bars show corresponding data for subsets isolated by CD77/CD10/IgD sorting into CD10−, CD77− non-GC cells that are IgD+ (□) or IgD− (), CD77+ centroblasts and CD10+ CD77− centrocytes (■). Viral loads are expressed as EBV genomes per 106 cells.