Blockade of KLRG1 causes an increased proliferation in CD8⁺ T cells. (A) Representative example of KLRG1 blockade measured after 3 days by ³H thymidine incorporation. (B) Representative example of KLRG1 blockade measured after 3 days by Ki67 staining. Plots shown are gated on CD8⁺ KLRG1⁺ T cells showing the percentage Ki67 staining within each group. (C) Pooled data showing proliferation of CD8⁺ T cells after E-cadherin antibody block. (D) Representative example of KLRG1 blockade in CD28/CD27 subsets measured after 3 days by ³H thymidine incorporation. (E) Pooled data showing the effect of E-cadherin antibody block on proliferative capacity in CD8⁺ CD28/CD27 T-cell subsets. Proliferation index was calculated by determining the ratio between cells proliferating in the presence of anti-CD3 stimulation versus E-cadherin blockade. All P values were calculated using the Student t test.