Improved migration of CD8⁺ T lymphocytes genetically modified to overexpress CCR4. (A) The expression of CCR4 by CTR and transduced CD8⁺ T cells, using FACS analysis. The data summarize the results of T-cell lines generated from 4 healthy persons. (B) A representative phenotypic analysis. Dotted lines indicate isotype control. (C) The migration of CTR () and CCR4⁺ (■) CD8⁺ T cells toward TARC gradients, using a transwell migration assay. The panel indicates that migration toward TARC is significantly increased when CD8⁺ T cells overexpress CCR4. The data are the mean ± SD for CD8⁺ T-cell lines generated from 4 healthy donors.