Relative NFAT1 protein and mRNA expression in stimulated UCB and AB CD4⁺ T cells. (A) Approximately 2 × 10⁶ isolated UCB and AB CD4⁺ T cells were stimulated in vitro as described for each of the designated time points and Western blot analysis performed. Data are representative of 5 independent experiments. (B) NFATc2 (NFAT1-encoding) mRNA was assayed in stimulated UCB and AB CD4⁺ T cells by qRT-PCR as described and normalized to UCB at 0 hours. Each bar indicates mean (± SEM) of 2 to 5 independent data points.