FVII minigene construct and modified U1-snRNAs. Schematic representation of the expression vector pSCFVII. The 37-bp repeats in the IVS7 are indicated by white boxes, and the asterisk represents the 5'ss cryptic site in the second repeat. The 5'ss consensus sequence and the 9726+5G>A change (▴) are shown at the top. The top part shows the complementary between the sequence of the mutated exon 7-IVS7 junction and the 5′ tail of the engineered U1-snRNA.