American Society of Hematology

Figure 5

$Figure 5. Analysis of the hepcidin-α2-M interaction using native FPLC. The main graph shows the UV absorbance of fractions of human plasma (broken line) and purified α2-M (broken line) separated by gel-size chromatography using FPLC (“Methods”). The inset shows that the complex of purified α2-M and 125I-hepcidin comigrates with the peak of radioactivity formed in blood plasma after addition of 125I-hepcidin. Free 125I-hepcidin (■); 125I-hepcidin incubated for 1 hour at 37°C with either plasma (▴; concentration 2.8 μM) or purified α2-M (●; 1.4 μM). Results are typical of 3 experiments.$

Analysis of the hepcidin-α₂-M interaction using native FPLC. The main graph shows the UV absorbance of fractions of human plasma (broken line) and purified α₂-M (broken line) separated by gel-size chromatography using FPLC (“Methods”). The inset shows that the complex of purified α₂-M and ¹²⁵I-hepcidin comigrates with the peak of radioactivity formed in blood plasma after addition of ¹²⁵I-hepcidin. Free ¹²⁵I-hepcidin (■); ¹²⁵I-hepcidin incubated for 1 hour at 37°C with either plasma (▴; concentration 2.8 μM) or purified α₂-M (●; 1.4 μM). Results are typical of 3 experiments.

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