IL-7 adjuvant treatment does not increase Melan-A–specific memory CD8⁺ T-cell responses on peptide immunization. Mice immunized with adjuvant/Melan-A_26-35 were either treated with rIL-7 () or left untreated (−) during the effector phase of the immune response (days 5-13). (A) Kinetics of Melan-A–specific CD8⁺ T-cell responses. The number of Tet⁺CD8⁺ T cells per milliliter of blood is shown. (B) In a separate experiment, mice were treated as in panel A. All mice received BrdU in the drinking water (days 5-13). Graphs represent the kinetics of BrdU incorporation in Tet⁺ (left panel) and in Tet⁻ (right panel) CD8⁺ T cells. The number of BrdU⁺ cells per milliliter of blood at the indicated time points is shown. Error bars represent SD (n = 5). The gray box in panel A indicates the IL-7 treatment period; and in panel B, the IL-7 and BrdU treatment period. *P < .05.