Tumor protection of RENCA cells in WT and different knockout BALB/c mice. (A) WT and indicated knockout BABL/c mice were vaccinated with 10⁶ irradiated RENCA cells and challenged 1 week later with 5 × 10⁶ RENCA tumor cells. The survival curve indicates percentage of mice that survived the challenge. The graphic represents data of 4 independent experiments (n = 4 mice/group). (B) Tumorogenicity of RENCA cells in different knockout mice. WT and indicated knockout BALB/c mice were inoculated on the back with 2 × 10⁶ RENCA tumor cells. Mice were killed when tumor size reached 15 mm in diameter or was ulcerated. This experiment is representative of 3 independent experiments. (C) Spontaneous production of GM-CSF by RENCA cells. Irradiated RENCA or B16-F10 cells (10⁶) were seeded in a 10-mm plate and GM-CSF release was detected by enzyme-linked immunosorbent assay (ELISA) from their supernatant after 24 hours. The graph is representative of 3 independent experiments.