Flow cytometric analysis of DC recruitment at the site of vaccination after B16 or B16-GM immunization. C57BL/6 WT (A), GM-CSF^−/− (B), IL-5^−/− (C), and βc^−/− (D) mice were immunized with irradiated B16 or B16-GM (10⁶ cells). Four days later cells at the vaccination sites were isolated and subsequently analyzed by flow cytometry for the presence of CD11c⁺, CD11b⁺, and CD8α⁺ DCs. Results are percentages and are representative of 1 of at least 3 animals per group. The difference observed between B16 and B16-GM in each group was highly significant (**P ≤ .005) except for the βc^−/− group (*P = .45).