Pre-miR-29b restores expression of hypermethylated ESR1 and p15^INK4b in the MV4-11 cell line. (A) Quan-titative RT-PCR for ESR1 in K562 and MV4-11 cells (B), and p15^INK4b (C) after 72 hours of nucleoporation with pre-miR-29b or scrambled oligonucleotides. Histograms represent mean values with SD. (D) Scatter plots of the quantitative DNA methylation data of ESR1 promoter regions in K562 (D) and MV4-11 cells (E) after nucleoporation with pre-miR-29b (■) or a scrambled oligonucleotide (●) obtained using the MassArray system.¹⁵  The lines connecting the dots indicate the corresponding CpG pairs. (F) Scatter plots of the quantitative DNA methylation data of p15^INK4b in MV4-11 cells after nucleoporation with pre-miR-29b (■) or a scrambled oligonucleotide (●). Each dot represents a single CpG or a group of CpGs analyzed. The lines connecting the dots indicate the corresponding CpG pairs. The scatter plots were created using GraphPad Prism, version 5.00 for Windows (GraphPad Software, San Diego, CA). The P values were obtained comparing the CpG methylation levels between scrambled and pre–miR-29b–transfected cells using paired Wilcoxon signed-rank test. Error bars indicate SD.