Roles of γδ T cells in naive T-cell proliferation in lymphopenic conditions. (A) Groups of TCRβ−/− recipients were injected with anti-Thy1.2 Ab (500 μg at days −2, 1, and 3) and subsequently transferred with 106 Thy1.1 naive CD4 and CD8 T cells (at day 0). Cells from the indicated tissues were harvested 7 days after T-cell transfer and stained for Thy1.1 and CD4. Dot plots are representative of 5 individually tested mice. (B) Total cell recovery of transferred T cells was calculated by FACS analysis; control Ig (●) or anti-Thy1.2 (○). (C) The ratio of donor T cells recovered from the spleen and mesenteric LN are shown. Each symbol represents individually tested mice from at least 2 independent experiments. (D) Groups of mice treated with anti-Thy1.2 Ab and transferred with naive T cells as described above were bled every week for 4 weeks and analyzed for relative expansion of each donor T-cell subset. The results are representative of at least 2 separate experiments involving 2 to 3 mice per group. (E) Ratio of CD4 and CD8 T cells in γδ T cells depleted (anti-Thy1.2) mice after 4 weeks was calculated. Each symbol represents individual recipients. *P < .05; **P < .01. (F) 3 × 106 γδ T cells were isolated from TCRβ−/− mice and transferred into groups of Rag1−/− recipients. Ly5.1 naive T cells were adoptively transferred into these mice 14 days after the γδ T-cell transfer. Expansion of Ly5.1 T cells was analyzed 7 days after the transfer (G) The ratio of donor CD4 and CD8 T cells in mice that received γδ T cells before naive T-cell transfer was calculated. The results are representative of 4 to 6 mice from 2 separate experiments. *P < .05; **P < .01. Shown are the mean ± SD of individually tested mice.
