Analysis of the CEACAM1⁺/Ly-6C^high/CD11b^high progenitor population and local angiogenesis after reverse transfer of CEACAM1⁺ and CEACAM1⁻ BM into recipient mice. (A,B) Summary of quantitative flow cytometric analyses of Ly-6C^high/CD11b^high populations in bone marrow (A) and peripheral blood (B) on day 21 after infection with L major after CEACAM1⁺ and CEACAM1⁻ BMT into B6.Ceacam1^−/− and B6.WT mice, respectively. Quantifications of Ly-6C^high/CD11b^high monocytic precursors after infection with L major and transfer of CEACAM1⁺ BM into CEACAM1⁻ recipients (rescue, ) and transfer of CEACAM1⁻ BM into B6.WT recipients (un-rescue, ▧) are shown. Data from B6.WT and B6.Ceacam1^−/− mice are depicted in ■ and □, respectively. Data are represented as means (± SEM) from at least 6 animals each, *P < .05; **P < .01. (C-F) Histologic analyses of cross-sections of infected footpads on day 21 after infection in H&E stainings (C,D) and immune fluorescence (E,F) after BM transplantation of CEACAM1⁺ BM into B6.Ceacam1^−/− mice (rescue; C,E) and CEACAM1⁻ BM into B6.WT mice (un-rescue; D,F). (E,F) Lymphatic vessels are shown in red (anti–LYVE-1 labeling) and blood vessels are colored in green (anti–MECA-32 labeling). Nuclei are shown in blue (DAPI). The white dotted line indicates the boundary between the skin and the inflammatory infiltrate. Magnification ×200. (G-I) Quantification of LYVE-1⁺ (G) and MECA-32⁺ areas (H) as well as cell-free spaces (I) in inflamed areas of infected footpads in control mice and after BM transplantation as indicated. *P < .05; **P < .01; ***P < .001.