Labeling of CEACAM1, LYVE-1, MECA-32, and nuclei in cross-sections of infected footpads. (A-C) Labeling of CEACAM1 (A, anti-CEACAM1 polyclonal antiserum P1; yellow) shows that the cellular infiltrate in the footpad is CEACAM1⁺. LYVE-1 is expressed on lymphatic vessels in the infiltrate (B, shown in red). In the overlay (C), coexpression of CEACAM1 and LYVE-1 on lymphatic vessels is shown in B6.WT mice. Blood endothelium (anti–MECA-32 labeling; green) also expresses CEACAM1 (C). In footpads from B6.Ceacam1^−/− mice, no CEACAM1 labeling is present (D), and LYVE-1⁺ structures (E) are CEACAM1⁻ (F). (G-I) After BM transplantation of CEACAM1⁺ BM into B6. Ceacam1^−/− mice (rescue), CEACAM1 labeling is restored in the infected footpads (G), and LYVE-1⁺ structures (H) coexpress CEACAM1 (I). In the un-rescue experiment after CEACAM1⁻ BM transplantation into B6.WT mice, CEACAM1-expressing cells are absent in the infiltrate (J) and lymphatic vessels (K,L). Representative photographs from 6 animals each are shown; magnification ×630.