Homing of intraperitoneally injected DCs to omentum. (A) Ex vivo imaging of pancreas from a NOD mouse 3 days after intraperitoneal injection of Luc⁺ DCs. Omental tissue was detached and turned aside between left and right photos to show that signal comes exclusively from omental and not pancreatic tissue. The tissue was imaged for 5 minutes. Representative data from n = 6 mice equally treated. (B) Percentage of CFSE⁺ cells in pancreas-associated omentum 2 days after intravenous or intraperitoneal injection of CFSE⁺ DCs. t test was used for statistical analysis. (C) Visualization of CFSE⁺ DCs in omentum by fluorescence microscopy 2 days after intravenous or intraperitoneal injection. Brightfield and fluorescence pictures are shown on the left and right side, respectively. Numeric aperture and time of exposure are indicated on the fluorescence picture.