Differential homing of BM-DCs within PLNs. (A) Ex vivo imaging of spleen, PLNs (PDLN vs GLN), and MLNs from NOD mice 2 to 3 days after intravenous or intraperitoneal injection of Luc⁺ DCs. Tissues were imaged for 5 minutes. Representative data from more than 10 mice. (B) Proliferation of BDC2.5 T cells, by CFSE dilution (x-axis), in PDLN, GLN, and RLNs, 3 days after intraperitoneal or intravenous injection. Data shown were gated on CD4 (y-axis). Data from CLNs and MLNs (not shown) were similar to RLNs.