Depsipetide treatment inhibited the cell proliferation and induced apoptosis in HTLV-I–infected cell lines. (A) The cells were treated with various concentrations of depsipeptide (0, 0.12, 0.5, 2, or 5 ng/mL), and the cell proliferation was measured 72 hours later by 3H thymidine incorporation. The data are shown as percentage of untreated control, represent the mean plus or minus SD of triplicates, and are representative of 3 independent experiments. (B) Induction of apoptosis by depsipeptide. The cells were treated with 2 ng/mL of depsipeptide for 24 hours or 48 hours, and the apoptotic cells were measured by annexin V staining. Data represent the mean percentage of apoptotic cells from 3 independent experiments. (C) Induction of caspase-3 and caspase-9 activities by depsipeptide. The data are representative of 3 independent experiments.
