SNX-2112 induces apoptosis in MM cells. (A) MM.1S, U266, and OPM1 cells were cultured for 48 hours with SNX-2112 at indicated doses. Apoptosis was determined by flow-cytometric analysis for Apo2.7 staining. (B) MM.1S, U266, and OPM1 cells were cultured with SNX-2112 (125 nM) for the indicated times, and with SNX-2112 for 24 hours at the indicated doses. Cell lysates were analyzed by Western blotting. FL indicates full-length; CF, cleaved form.