PRELI is up-regulated in response to activation in human primary T helper cells at the mRNA and protein level. (A) Expression of PRELI mRNA. Naive CD4⁺ cells were activated and cultured in the absence (Th0) or presence of IL-12 (Th1) or IL-4 (Th2). The figure represents the fold change of PRELI expression in Th0, Th1, and Th2 cells versus nonactivated (Thp) cells (Affymetrix probe set (224232_s_at). Representative data from 3 independent experiments. (B) Expression of PRELI at protein level. Naive CD4⁺ cells were cultured as in panel A for 6 and 24 hours. Samples from 5 donors (neonates) were pooled.