Prediction and validation of cis-regulatory modules for Lmo2. CRMs were predicted both by their conservation from mouse to the marsupial opossum (a measure of sequence constraint is shown on the last line of the locus map) and by peaks of acetylation of histone H3 in the chromatin. The CRMs with demonstrable activity in transgenic mice are shown as blue boxes on the first group of lines on the map (pPex is the extended proximal promoter), followed by the locations of introns and exons of the Lmo2 gene and a distal promoter. The regulatory potential³  score (light blue data track) has peaks in most of the CRMs, but some noncoding regions with strong constraint and regulatory potential are not active in the transgenic mouse assay. Below the locus maps is a summary of the occupancy of the CRMs by transcription factors and the pattern of expression that they regulate. See the complete figure in the article beginning on page 5783.