EGCG neutralizes the cytotoxic effects of BZM in glioblastoma. (A) Percentage cell viability of LN229 cells treated for 48 hours with increasing concentrations of BZM in the presence of increasing concentrations of EGCG was determined via MTT assay. (B) U251 and LN229 were treated with 10 nM BZM in the presence or absence of 10 or 20 μM EGCG for 48 hours and the number of long-term surviving cells (2 weeks) was determined by colony-formation assays. Images were taken of representative 6-well plates. (C) The average number of colonies of U251 and LN229 cells treated with BZM in the presence or absence of EGCG is presented as the percentage of colonies from untreated cells, which was set at 100% (mean ± SE; n ≥ 3).