Figure 2
Assays of cultures of viable MSCs passage 1/donor 5 that were plated at 100 cells/cm2 and incubated for 5 days or 9 days to generate passage 2 MSCs. To prepare passage 3 MSCs, 9-day cultures were lifted with trypsin/EDTA and replated at 100 cells/cm2 for incubation for 5 or 9 days. (A) RT-PCR assays. (B) Western blot assays. (C) Assays by immunocytochemistry. Bar = 200 μm. (D) Double-immunostaining for PODXL (red) and the 5 other surface proteins (green). Nuclei were labeled with 4′,6-diamidino-2-phenylindole (blue). (Top panels) Bar = 200 μm. (Bottom panels) Bar = 50 μm. Arrows indicate regions of the cells in which PODXL and other proteins are colocalized.
