Assays of MSCs in vitro. Viable MSCs from passage 1/donor 1 plated at 100 cells/cm2 were incubated for 5 or 9 days to generate passage 2 MSCs that were high or low for PODXL or CD49f. (A) Passage 2/donor 1 MSCs replated at 1 cell/cm2 and incubated for 2 weeks. Colonies were stained with crystal violet. (B) CFU-F values passage 2/donor 1 and donor 8 MSCs (donor 7009 and 281). Asterisks indicate values (± SD) that differ with P < .01 by the Student t test (n = 7). (C) Alizarin Red S after osteogenic differentiation and after adipogenic differentiation with passage 3/donor 1 MSCs. Bar = 100 μm. (D) Assays of differentiation by extraction of Alizarin Red S after osteogenic differentiation and Oil Red O after adipogenic differentiation with passage 3/donor 1 MSCs. Asterisks indicate values (± SD) that differ with P < .01 by the Student t test (n = 4).
