American Society of Hematology

Figure 3

$Figure 3. Myeloid potential of MPPs after PTX treatment. (A) FACS analysis of bone marrow cells from irradiated recipient WT mice 48 hours after intravenous injection of GFP+ VCAM-1− MPPs with (right panel) or without (middle) PTX treatment. Bone marrow from mice that did not receive an injection of cells is also shown as a control (left). Shown FACS plots are pregated on the Lin− fraction. The percentages represent the frequency of GFP+-injected cells in whole bone marrow. In vivo (B) and in vitro (C) GM differentiation potential of VCAM-1+ and VCAM-1− MPPs with or without PTX treatment. After 2-hour culture in the presence or absence of PTX, the cells were subjected to the experiments as described in Figure 1C,D. *P < .05 (statistical significance) by Student t test.$

Myeloid potential of MPPs after PTX treatment. (A) FACS analysis of bone marrow cells from irradiated recipient WT mice 48 hours after intravenous injection of GFP⁺ VCAM-1⁻ MPPs with (right panel) or without (middle) PTX treatment. Bone marrow from mice that did not receive an injection of cells is also shown as a control (left). Shown FACS plots are pregated on the Lin⁻ fraction. The percentages represent the frequency of GFP⁺-injected cells in whole bone marrow. In vivo (B) and in vitro (C) GM differentiation potential of VCAM-1⁺ and VCAM-1⁻ MPPs with or without PTX treatment. After 2-hour culture in the presence or absence of PTX, the cells were subjected to the experiments as described in Figure 1C,D. *P < .05 (statistical significance) by Student t test.

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