Expression of EGFP in Tg(runx1P1:EGFP)–transgenic embryos. (A–D) bright-field images, (A′–D′,D″) fluorescent images, (B″) merge of B and B′, and (D‴) merge of D′ and D″. (A,A′) Lateral views at 18 hpf showing EGFP in the posterior LPM (arrowhead) and the olfactory placode (arrow). (B,B′) Confocal images of the LPM showing EGFP-positive hematopoietic cells. (C,C′) Lateral views of 24 hpf embryo with EGFP expression in the PBI (arrowhead), olfactory placode (arrow), and hypochord. (Inset) Confocal images displaying EGFP-positive cells in the PBI (arrowhead). (D,D′,D″,D‴) Lateral view of the PBI of a 24 hpf Tg(runx1P1:EGFP)/Tg(gata1:DsRED) double-transgenic embryo, showing coexpression of EGFP and DsRED in EMPs.