Figure 5
Effect of lactadherin on the phagocytosis of leukocytes and endothelial cell–derived microvesicles. BODIPY-maleimide–labeled microvesicles from peripheral blood leukocytes or human umbilical vein endothelial cells were incubated with THP-1–derived macrophages (106 cells/well) for 30 minutes with or without lactadherin (0.8 μg/mL), and the extent of phagocytosis was quantified by flow cytometry by measuring the percentage of green fluorescence (BODIPY)–positive macrophages as in Figure 3.
