Figure 8
![Figure 8. Thrombin generation in the plasma of lactadherin-deficient mice and control littermates. The reaction mixture consisted of plasma (80 μL) and the trigger solution (3 ng purified Russell viper venom in 40 μL), fluorogenic thrombin substrate Z-Gly-Gly-Arg-AMC (2.5 mM), and CaCl2 (10 mM). The generation of thrombin was measured as a function of fluorescence in a fluorimeter over a period of 30 minutes. Thrombin was then calculated using an α2-macroglobulin thrombin complex standard as described by Hemker et al.27 Representative data are presented. Blood samples from 5 mice from each group (lactadherin−/− and littermate controls [lactadherin+/+]) were pooled for each experiment.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/113/6/10.1182_blood-2008-07-167148/4/zh80060930840008.jpeg?Expires=1735500858&Signature=X8bm-aK7S3wdUtQT6OE0ss3mCNqbUCHZz4kAaXcSqHOkCiGnG3BwzrrfERQk0dIONoVuvBjdpFLkCQzddcs1Qh0ISiCsfRP66ekIixoKBLk3CRy1J2aHbeD2-9P022TT-nS3mph6ZrEk4vvfhSolXJ7Ewni6k5gElO1j89VJWHRWZ3jY-BUqlIX9qJ8wLZ8ucYGkIG2dPGPtdfUThPSwQG4-x4ZbfrwhJQ3vE6PZiJ4mQOniPpnT4e-D6a8ulYX~B9HsWZd~bZtgzVnBsiijBrtka7C3Z6CjIspAUyaTqBROAkMJPn93f-qgDzuYWSUoupg7R8dKp78pT9uqObEZlA__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Thrombin generation in the plasma of lactadherin-deficient mice and control littermates. The reaction mixture consisted of plasma (80 μL) and the trigger solution (3 ng purified Russell viper venom in 40 μL), fluorogenic thrombin substrate Z-Gly-Gly-Arg-AMC (2.5 mM), and CaCl2 (10 mM). The generation of thrombin was measured as a function of fluorescence in a fluorimeter over a period of 30 minutes. Thrombin was then calculated using an α2-macroglobulin thrombin complex standard as described by Hemker et al.27 Representative data are presented. Blood samples from 5 mice from each group (lactadherin−/− and littermate controls [lactadherin+/+]) were pooled for each experiment.
