Presence of JAK2V617F in the ECs lining the terminal hepatic venules in the sections of archived liver biopsy tissue specimens from BCS patients. (A) The ECs from a terminal hepatic venule with less congestion from a liver biopsy of a patient with BCS with PV captured by LCM after hematoxylin and eosin staining (top panel) or immunohistochemical staining with an anti-CD34 antibody (bottom panel). These photomicrographs show a portion of a terminal hepatic venule lined by ECs. There is a thin rim of underlying connective tissue in the subendothelial space. The boxed or underlined area shows longitudinally sectioned endothelial cells. The rest of the photo shows plates of hepatocytes arranged in trabeculae. The right panel demonstrates the same areas after laser capture of the ECs. The remaining adjacent tissue remains intact (original magnification ×400; ArcturusXT System (MDS Analytical Technologies). (B) JAK2V617F status of LCM captured hepatocytes (H) and endothelial cells (EC) of patients by allele-specific nested PCR for JAK2V617F. (C) Table summarizing the transcripts present in hepatocytes (H) and ECs isolated by LCM from archived liver biopsy tissue specimens. The numbers represent the average CT values of the samples, which were run in duplicate and repeated on 3 separate occasions.