RNAi of cytohesin-1 reduces chemotaxis of mature Mo-DCs. Transwell migration assays show that CCL19 (200 ng/mL)–induced chemotaxis is overall strongly reduced in cytohesin-1 knockdown mature Mo-DCs, using integrin ligands ICAM-1-Fc (∼ 10 μg/mL; A), fibronectin (50 μg/mL; B), collagen I (30 μg/mL; C), or uncoated filters (D). Overexpression of wild-type cytohesin-1 but not of the GEF-deficient E157K mutant increases chemotaxis of GFP-cotransfected Mo-DCs (uncoated filters; E). Error bars indicate ± SD. *P < .05. Each experiment was repeated at least 3 times independently. Each single experiment was performed in duplicate.