GAS systemic spread in Fga^−/− and Fga^+/− mice infected with UMAA2616. Bacterial counts from spleens of 8 Fga^−/− and 9 Fga^+/− mice inoculated with 2 × 10⁶ CFU UMAA2616 were calculated by a plating method. Data were pooled from 2 independent experiments (2.8 × 10⁶ and 2.0 × 10⁶ CFU). The difference of CFU between Fga^−/− and Fga^+/− mice was tested by the Mann-Whitney test. P value is indicated.