Enhancement of HIV replication by TLR2 ligation in mLCs is dependent on the TLR2-MyD88 signal pathway. After HIV exposure, mLC were preincubated with anti-TLR2 mAb (10 μg/mL) for 30 minutes or MyD88 inhibitory peptide (100 μM) for 24 hours, followed by coculturing with 5 × 10⁸/mL HKLM, or 5 μg/mL PGN for 24 hours. Isotype control or control peptide experiments were performed using the same conditions. HIV-infected mLCs were assessed 7 days later by HIV p24 intracellular staining. (A) Anti-TLR2 mAb; (B) MyD88 inhibitory peptide. Summary of experiments and representative FACS analyses of CD11c and p24 mAb double-stained cells are shown. Results are shown as means plus or minus SD (n = 3). *P < .05. All data shown represent at least 2 separate experiments.