TLR2, but not TLR3, stimulation negatively modulates A3G expression levels in mLCs. mLCs and mDCs were cultured in medium alone or with 5 × 10⁸/mL HKLM, 5 μg/mL Pam₃CSK₄, or 20 μg/mL poly(I:C) for 24 hours before HIV exposure. Expression of A3G in mLCs and mDCs was assessed using qPCR (A) or Western blotting (B). Results are shown as means plus or minus SD (*P < .05; **P < .01). Alternatively, cells were lysed and centrifuged at high speed, with equal volumes of the P fraction and the SN fraction, and then analyzed for A3G expression by Western blotting. A3G expression was quantified, with a value of 1 assigned to the pellet fraction in nontreated cells (C). All data shown represent at least 2 separate experiments.