Expression of Hedgehog family members in the spleen. (Top) RT-PCR analysis of Ihh, Shh, and Dhh in the spleen at the indicated times after induction of acute anemia by PHZ injection. C indicates positive control RT-PCR analysis for Ihh, Dhh, and Shh. Dhh control used spleen RNA, and Ihh and Shh controls used thymus RNA. (Bottom) β-Actin control RT-PCR was used as a loading control. C1 indicates Dhh control RNA; C2, control RNA for Ihh and Shh.