Blocking integrin αV function inhibited HIV-1 replication in HeLa-P4R5-MAGI cells. (A) RNAi mediated inhibition of αV and β5 integrins. Flow cytometry dot plots of αV (vertical axis) and β5 (horizontal axis) of mock-treated cells or treated with different siRNAs (siAlphaV#1, siAlphaV#2, siBeta5, and siNontargeting as a control) 48 hours after transfection. One representative experiment is shown. (B) Inhibition of HIV-1 replication in αV-silenced and β5-silenced HeLa cells. Percentage of HIV-1 NL4-3 replication compared with the mock control is shown. Data are mean plus or minus SD of at least 3 independent experiments. (C) S36578 inhibition of viral replication of different HIV strains. Percentage of replication (mean ± SD of at least 3 independent experiments) at different S36578 concentrations for each of the viruses used is shown. *P < .05; **P < .005; ***P < .001.