APC populations expressing a similar phenotype to cutaneous migratory APCs were detected in the paracortex of the human axillary lymph node. Frozen lymph node sections were probed with antibodies detecting CD3 and CD21 to identify the T lymphocyte–rich paracortex and follicles, respectively (A). Immunohistochemistry illustrated that a subset of CD1a⁺ APCs in the paracortex (B) coexpressed CD207 or Langarin (C). CD207⁺ APCs were either concentrated in the diffuse T-lymphocyte zones surrounding dense T-lymphocyte regions (B,C) or distributed throughout areas densely packed with T lymphocytes (D). Proportions of both CD1a⁺CD207⁻ and CD1a⁺CD207⁺ APCs coexpressed CD208/DC-LAMP; however, not all CD208⁺ cells expressed CD1a or CD207 (E). APCs expressing CD1a or CD208 did not coexpress CD68 (F). Differing expression patterns of CD1a, CD207, and CD208 can therefore be used to distinguish between 3 APC populations in the paracortex: CD1a⁺CD207⁻CD208^+/−, CD1a⁺CD207⁺CD208^+/−, and CD208⁺CD1a⁻CD207⁻ APCs (G). Subsets of CD1a⁺CD207⁻ and CD1a⁺CD207⁺ APCs (H) and the majority of CD1a⁻CD208⁺ APCs (I) expressed the lipid presentation molecule CD1b. Cells expressing CD1b but no CD208 or CD1a were rare (I). Panels A to C, E, F, and I were acquired from the same area on sequential sections. The images shown in panel H were acquired from different fields. *Background autofluorescence. Data are representative of 5 independent experiments.