Anti-CD3 preconditioning inhibited donor T-cell expression of homing and chemokine receptors. Five days after HCT, donor T-cell expression of gut homing α4β7 receptor and CCR9 in MLNs, donor T-cell expression of skin homing E-Lig, P-Lig, CCR4, and CCR10 in PLNs, and donor T-cell expression of non–tissue-specific CCR5 and CXCR3 in spleen were compared. There were 4 recipients in each group. (A) A representative FACS pattern of CCR9 and α4β7 receptor by gated H-2b⁺CD4⁺ or H-2b⁺CD8⁺ donor T cells from MLN, as well as a representative FACS pattern of CCR4, CCR10, E-Lig, and P-Lig of donor CD4⁺ or CD8⁺ T cells from PLN. (B) Mean plus or minus SE of CCR9⁺ or α4β7⁺ cells among donor CD4⁺ or CD8⁺ T cells from MLN. (C) Mean plus or minus SE of CCR4⁺, CCR10⁺, E-Lig⁺, or P-Lig⁺ cells among donor CD4⁺ or CD8⁺ T cells from PLN. (D) A representative FACS pattern of CCR5 and CXCR3 by gated H-2b⁺CD4⁺ or H-2b⁺CD8⁺ donor T cells from spleen. (E) Mean plus or minus SE of CCR5⁺ or CXCR3⁺ cells among gated donor CD4⁺ or CD8⁺ T cells from spleen of 4 recipients.