Effect of G-CSF and dexamethasone on granulocytes. (A) Comparison of the granulocyte concentration per milliliter of blood and the EMR3 expression level in time, after donor treatment with G-CSF and dexamethasone (representative graph). PMNs indicate polymorphonuclear leukocytes; ○, EMR3 expression; ■, PMNs per milliliter. (B) Changes in granulocyte gene expression after stimulation with G-CSF and dexamethasone. At 18 to 20 hours after stimulation of the donors or culture of granulocytes with G-CSF and dexamethasone in vitro, neutrophil gene expression was determined by the use of Agilent Whole Humane Genome microarrays. Numbers refer to differentially expressed genes in neutrophils isolated 18 to 20 hours after the administration of G-CSF and dexamethasone in vivo and/or those cultured overnight in medium supplemented with G-CSF and dexamethasone.