PfEMP1 is correctly trafficked to MCs in Pf332 KO lines. Immunofluorescence analysis of 3D7 or 3D7ΔPf332 (11G7 or 13E2) iRBCs with anti-VARC antibody shows punctate PfEMP1 fluorescence consistent with its localization in MC. Double labeling with anti-VARC (green) and anti-SBP1 (red) antibodies shows coresidence of PfEMP1 in some (but not all) of SBP1-positive MCs. A total of 2 representative RBCs are shown for each transgenic parasite line and show no detectable difference between the intracellular localization of PfEMP1 in 3D7 parasites or either of the Pf332 KO clones.