Detection of α-spectrin gene/^Aγ-globin mRNA in reticulocytes of transgenic mice. RNA from adult reticulocytes was hybridized to ³²P-labeled antisense riboprobes for the human ^Aγ-globin gene (top panel) and the mouse α-globin gene (bottom panel) and digested with RNase. Protected fragments were separated by polyacrylamide gel electrophoresis followed by radiography. Representative transgenic lines are shown, (A-F) with the letters above the lanes corresponding to Table S3. Autoradiography time of the exposure shown was 20 hours for the γ-globin probe and 5 hours for the α-globin probe, respectively.