Elevated levels of IL-31 elaboration by MPD HCMCs. (A) HCMCs (105/mL) from 19 MPD patients and 8 Gmob volunteers were sensitized with IgE overnight and then were challenged with or without anti-IgE for 30 minutes at 37°C. A separate aliquot of MCs (105/mL) was stimulated with substance P (Sub P) for 30 minutes. The levels of IL-31 in the supernatants were measured by ELISA. (B) HCMCs (106/mL) were incubated in serum-free media for 24 hours; total RNA was then extracted from each MC population. The levels of IL-31 in the conditioned media were measured by ELISA. Each column represents the mean ± SD of the levels of IL-31 in HCMCs from each group. (C) The IL-31 mRNA expression was quantitated by measuring the threshold cycle and presented as relative rates by comparing with the expression of the reference gene β-actin. The data were analyzed by 2−ΔΔCT method.
