KGF produces increased CD8⁺ T-cell responses to DNA tumor vaccine without increased CD4⁺Foxp3⁺ cells in day 30 recipients of allogeneic BMT. B6 mice underwent T cell–depleted BMT and were treated with KGF as described in Figure 1. On days 14, 19, and 24, mice were vaccinated with VP22-opt-TRP1 plasmid DNA. On day 30, the numbers of CD8⁺tetramer⁺ cells (A, *P = .04), CD4⁺Foxp3⁺ cells (B), and ratio of CD8⁺tetramer⁺ to CD4⁺Foxp3⁺ cells (C, *P = .01) from spleens were determined by flow cytometry. Whole splenocytes from transplanted groups also were stimulated overnight in the absence or presence of wild-type murine TRP1 455 peptide, and intracellular production of IFN-γ and TNFα by CD8⁺ cells was determined by flow cytometry (D, *P = .04, **P = .009, ***P = .03, ****P = .01). Data are pooled from 2 experiments with similar results.