ChIP-chip assays across 250 kb of the mouse Lmo2 locus reveal candidate distal regulatory elements. ChIP-chip analysis of histone H3 acetylation in 11 hematopoietic and endothelial cell types. MVista representation of mouse/human sequence conservation is shown on the top and annotations of promoters (gray), and all candidate regulatory elements (black) are as in Figure 1, except that regions not enriched (−88, −58, −47, −43, −3, and +7) are marked by dotted lines. Enrichment cluster I comprises −90, −75, −70, −64; cluster II covers −40, −35, −25, −12, +1. The y-axis represents the log 2 enrichment of ChIPed DNA over input DNA ranging from −1 to 5, whereas the x-axis depicts the 250 kb spanning the mouse Lmo2 locus, where the translation initiation codon (ATG) is marked as position 0. The black bar on the right hand side indicates relative levels of Lmo2 expression in the 11 different cell types. The cells surveyed included non-Lmo2–expressing ES cells, as well their in vitro–differentiated EB sorted for Brachyury⁻/Flk1⁻ (B⁻/F⁻: premesoderm), Brachyury⁺/Flk1⁻ (B⁺/F^−: prehemangioblast mesoderm), and Brachyury⁺/Flk1⁺ (B⁺/ F⁺: hemangioblast mesoderm). Additional cell types included Lmo2-expressing cell lines, representing endothelial progenitor MS1, multipotential hematopoietic progenitor HPC7, myeloid progenitors 416B, erythroid progenitor MEL, and primary cells derived from day 11.5 FL. In addition, cell types were used, in which Lmo2 expression is supposed to be extinguished, such as a T-lymphoid cell line (BW) and whole adult murine thymus.