Figure 2
Expression of transgene-encoded VWF in VWD BOECs after lentiviral transduction. VWD BOECs were isolated from dogs with VWD type 3 and transduced with lentiviral vectors encoding full-length human VWF. VWF immunostaining revealed high concentrations of VWF present in both the cytoplasm and Weibel-Palade bodies (magnification) of transduced VWD BOECs (Lenti-CMV-huVWF) but not in VWD BOECs transduced with empty lentiviral particles (empty vector). Reprinted from De Meyer et al68 with permission.
