HIV Gag-specific IFNγ and IL-4 ELISpots from isolated splenocytes. (A) Effects of cytokine adjuvants on the induction of a Th1 response were measured via the use antigen-specific IFNγ ELISpots performed on isolated splenocytes. ELISpots were performed on splenocytes harvested from mice that received IL-12 as an adjuvant or IL-28B as an adjuvant (n = 4) and IFNγ spot-forming units (SFU) were counted. (B) Effects of cytokine adjuvants on the induction of a Th2 response were measured in the same fashion using IL-4 ELISpots.