Expression of uPA, α-granule proteins, vinculin, and CAMK2G in QPD (Q) and control (C) CD34⁺ cells, cultured megakaryocytes, and platelets. (A) RT-qPCR analysis of uPA, VWF, vinculin, and CAMK2G mRNA levels in platelets and/or CD34⁺ cells, and day-7 and -13 megakaryocytes, relative to controls (arbitrarily set to 1) to identify altered patterns of gene expression in QPD. (B) Comparison of uPA, PF-4, TSP-1, and VWF antigen in megakaryocyte culture supernatants, evaluated by ELISA at different stages of culture. Undetectable levels are presented as zero. Data represent mean values; error bars indicate SEM for data from 3 to 5 subjects. *Significant increases (P < .05) in QPD, compared with control.