Figure 4
Large-scale production using the GPRG-TL20-GFP cell line. A 5-liter WAVE Bioreactor was seeded with 5 × 108 cells at day 0 and cultured for 2 weeks. At day 7, cells were washed free of doxycycline. Aliquots of media were harvested daily and assayed for infectious vector (left axis) and p24 antigen, from which the net daily gain in p24 concentration was calculated and plotted (right axis).
