A subset of VWF strings binds platelets. Perfusion assays were performed in the presence of 100 μM histamine, 108/mL fixed platelets, and fluorescent VWF polyclonal antibody. (A) A representative image of VWF strings and platelet strings for perfusion assay conducted at 2.5 dyne/cm2. Bar represents 10 μm. (B) Total fluorescent VWF strings (■) and platelet-decorated VWF strings (□) formed under a fluid shear stress of 2.5, 10, and 20 dyne/cm2 are shown as the mean plus or minus SEM from 10 fields per experiment. (C) Perfusion assays were performed at a shear stress of 2.5 dyne/cm2 in the presence or absence of 20 μg/mL antiplatelet GPIbα antibody 6D1. Total fluorescent VWF strings (■) and platelet-decorated VWF strings (□) are shown as mean plus or minus SEM from 10 fields per experiment. Each experiment was repeated at least twice.
