Figure 7
Figure 7. Integrin αv decorates acutely secreted VWF strings. Confluent HUVECs in a flow chamber were stimulated with 100 μM histamine at 2.5 dyne/cm2 shear stress for 5 minutes, fixed, and incubated with anti-VWF and anti-integrin αvβ3 antibodies as described in “Immunofluorescence microscopy.” Panels show immunofluorescence for VWF (A,D) and integrin αvβ3 (B,E) with corresponding fluorescent antibodies. Merged images are shown in panels C and F. Arrows indicate selected examples of the colocalization of VWF and integrin αvβ3. Bars represent 10 μm.

Integrin αv decorates acutely secreted VWF strings. Confluent HUVECs in a flow chamber were stimulated with 100 μM histamine at 2.5 dyne/cm2 shear stress for 5 minutes, fixed, and incubated with anti-VWF and anti-integrin αvβ3 antibodies as described in “Immunofluorescence microscopy.” Panels show immunofluorescence for VWF (A,D) and integrin αvβ3 (B,E) with corresponding fluorescent antibodies. Merged images are shown in panels C and F. Arrows indicate selected examples of the colocalization of VWF and integrin αvβ3. Bars represent 10 μm.

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