Retroviral infection with SWAP-70-GFP increases surface localization of MHCII molecules in SWAP-70–deficient DCs. Seven-day CD11c⁺ BMDCs were transfected with SWAP-70-IRES-GFP (SWAP-70-GFP) or IRES-GFP (empty vector) by retroviral infection. As control, cells were mock-treated (noninfected). The average of mean of the CD11c⁺MHCII^high BMDCs population is shown with plus or minus SD. For the SWAP-70-GFP and empty vector, the GFP⁺CD11c⁺MHCII^high BMDCs population is shown. Representative data of 3 independent experiments are shown. White columns indicate wt untreated; black columns, wt and LPS; dotted columns, SWAP-70^−/− untreated; and striped columns, SWAP-70^−/− and LPS as indicated. *P < .001.