SWAP-70 interacts with active Rac1 and RhoA in DCs. (A) SWAP-70^+/+ BMDC lysates were immunoprecipitated with different Rho-GTPases antibodies. Complexes were analyzed by Western blotting with anti–SWAP-70 (top) or anti-RhoGTPase antibody (bottom). (B,C) Colocalization analysis of SWAP-70 (green) and RhoA or RhoB (red). (B) Colocalization images show gray spots representing interaction of the 2 proteins. An area around the cytoplasm (yellow line) was drawn for quantification of gray values. Bar represents 10 μm. (C) Histograms (top panels) show the distribution of gray values for the colocalization image for SWAP-70-RhoA and SWAP-70-RhoB. (D) The average of the mean gray values obtained from all of the histograms (30 for each staining) analyzed. *P < .001.